Filename 62-1_POS01.CDF Data format NetCDF Author Dr. Fumio Matsuda Institute RIKEN CSRS, Japan Date submitting 2013/6/10 Species Oryza sativa Genotype Norin29:NIAS_accession_No.08-7131 Organ Aerial part Biosource amount (mg) 114.9 Growth support Bonsol II; Sumitomo Chemical, Tokyo Growth location Green house Growth plot design approx. 30 seedlings in 250 (pi) x 300 mm pot Light 12-h light /12-h dark cycle Humidity 80% Temperature 12-h 28 C/12-h 20 C cycle Watering regime kept under constant subirrigation conditions using tap water Nutritional regime no treated Date(s) of plant establishment 16/7/2009 Other specific metadata Nothing. Biotic treatment no treated Abiotic treatment no treated Intervention treatment no treated Treatment dose or intensity levels no treated Treatment time, time intervals and duration before harvest no treated Harvest date, time 31/7/2009 Plant growth stage 15 days Metabolism quenching method Freezing with liquid N2 Harvest method Time from tissue resection to liquid N2 freezing was one minute. Tissues were pooled in ependorf tube. Sample strage Stored at -80C for more than one month Reprecate sampling and analyses n=2-4 Tissue harvesting method Time from tissue resection to liquid N2 freezing was one minute. Tissues were pooled in ependorf tube. Tissue processing method no processing Storage condition prior to extraction or further processing Stored at -80C for more than one month Extraction method The frozen tissues were homogenized in 5 volumes of 80% aqueous methanol containing 0.5 mg/l of lidocaine and d-camphor sulfonic acid (Tokyo Kasei, Tokyo, Japan) by using a mixer mill (MM 300, Retsch) with a zirconia bead for 6 min at 20 Hz. Extract cleanup and/or Additional manipulation Following centrifugation of 15000 g for 10 min and filtration (Ultrafree-MC, 0.2 ?m, Millipore, Bedford, MA, USA) Extract storage and/or relocate Stored at 4 degree for 4 - 48 hours Chromatography instrument description Waters, Acquity UPLC system, operated by MassLynx4.1 Auto-injector Waters, Acquity SM, MassLynx4.1, 2microL injection, 1 wash per injection (600 microL water and 200 microL acetonitrile) Separation column and pre/guard column Waters Acquity BEH C18 (pore size: 1.7 microm) 2.1 by 100 mm with guard column (Waters, VanGuard 2.1 by 5 mm) Separation parameter solvent system: acetonitrile (0.1% formic acid):water (0.1% formic acid); gradient program: 1:99, v/v, at 0 min; 1:99, v/v, at 0.1 min; 99.5:0.5 at 15.5 min; 99.5:0.5 at 17.0 min; 1:99, v/v, at 17.1 min; and 1:99 at 20 min; flow rate: 0.3 ml/min; column temperature: 38 C. Instrument description Waters, Q-Tof Premier, operated by MassLynx4.1 Sample introduction and delivery From LC Ionization source ESI positive. capillary voltage: +3.0 keV; cone voltage: 22.5 V; source temperature: 120 C; desolvation temperature: 450 C; cone gas flow: 50 l/h; desolvation gas flow: 800 l/h; collision energy: 2 V; Mass analyzer description and acquisition mode quadrupole-time-of-flight operated at scan mode Data acquisition parameters detection mode: scan (m/z from 100 to 2000; dwell time: 0.45 s; interscan delay: 0.05 s, centroid). Lock spray was not employed (mass accuracy: ca. 5ppm, resolution 8500) Data file format NetCDF converted from .RAW data by using DBridge (Waters)