MeKO Analysis Results

Information:
Allele Symbol pad4-1
AGI Code AT3G52430
Gene Name ARABIDOPSIS PHYTOALEXIN DEFICIENT 4 / PHYTOALEXIN DEFICIENT 4
Description "Encodes a lipase-like gene that is important for salicylic acid signaling and function in resistance (R) gene-mediated and basal plant disease resistance. PAD4 can interact directly with EDS1, another disease resistance signaling protein. Expressed at elevated level in response to green peach aphid (GPA) feeding, and modulates the GPA feeding-induced leaf senescence through a mechanism that doesn't require camalexin synthesis and salicylic acid (SA) signaling. Required for the ssi2-dependent heightened resistance to GPA."
Size/Weight nomal
Color nomal
Shape nomal

 

Analysis results:
Normalization CRMN
Differential Accumulation limma
MS Type GC-TOF-MS
Visual phenotype
Metabolite profile
Red: significantly increase, Blue: significantly decrease (log2FC >= |1| and FDR < 0.05)
Replicate quality check
Volcano plot

blue-colored name: top 30 metabolites to be highlited.
Top 30 metabolites
ID logFC t P.Value adj.P.Val B
l-isoleucine -1.36269439697539 -6.02723412668797 2.89432248661106e-09 6.97531719273265e-07 10.6895775753252
succinic acid 1.39054293707961 5.35344307257704 1.23155528054366e-07 1.48402411305511e-05 7.09432737445826
succinate-d4 0.756211732020923 5.1030312572238 4.49555194137464e-07 3.61142672623763e-05 5.85885656182366
l-lysine -1.33470932891271 -4.60479899127847 5.0394122005875e-06 0.000303624585085397 3.56376941057032
l-tyrosine -1.11066150098 -4.25949228401936 2.37864701766195e-05 0.00114650786251306 2.10034670590506
l-aspartic acid 0.643481610050522 3.93634237870826 9.22908180346954e-05 0.0037070145243936 0.830165438061841
U -1.79983167045071 -3.87191599936335 0.000122455860990164 0.00421598035694707 0.572986934212563
U 3.75812647319189 3.71605730370888 0.000227464617668902 0.00685237160727566 0.313876174318068
U -0.898178388454268 -3.59435174542207 0.000351677633241632 0.00906011631980391 -0.41221326403998
U 0.668517704084557 3.57650401124631 0.000375938436506386 0.00906011631980391 -0.473885723874634
U -1.29129596645948 -3.49309049375161 0.000513012609212126 0.0112396398927384 -0.760029254078107
U 0.808432860390631 3.31534427480387 0.000970391340195213 0.0194886927489205 -1.34537187968193
U 0.451268785721041 3.17577891138771 0.0015699345077247 0.0291041704893579 -1.78468996143882
glycine 1.39328291894 3.11271563967356 0.00195092329832953 0.0335837510641012 -1.97897423196558
hydroxylamine 0.61830563650593 3.03945546045141 0.00247183759203681 0.0397141906453914 -2.1963728978441
U 0.58764761523501 2.96375297469141 0.00315838435914657 0.0468517141740612 -2.41750335494704
l-threonic acid 0.885086889726868 2.94972299703708 0.00330489270107485 0.0468517141740612 -2.45790198501104
1104:threonic acid-1,4-lactone 0.707857131058475 2.90727036464697 0.00378083211475973 0.0506211410920609 -2.57891616162991
stearic acid 1.12605337310955 2.84525354200449 0.00459398919170266 0.0582711260631759 -2.75284179974143
l-glutamic acid 0.473544626597505 2.78558549690024 0.00550958486339007 0.0663904976038504 -2.91627771587032
U -1.21009079308468 -2.74892330063991 0.00618611486299427 0.0709930324753152 -3.01593938476474
U -0.932199095970294 -2.72858937714078 0.00654940820480007 0.0717457898798553 -3.06960241790922
U 1.30577652878935 2.52812279238376 0.0117342998051948 0.122955054480519 -3.46048093407773
U -1.20225286139733 -2.46933609291285 0.0138251384112871 0.129601299711484 -3.60333383307662
citric acid 0.693328391576735 2.47790855721374 0.0134903465094141 0.129601299711484 -3.70659148349069
l-leucine -0.614587377494201 -2.46489427621971 0.0139818829564257 0.129601299711484 -3.73815710247216
U 1.1453428504319 2.43600420232129 0.0151534571794295 0.135258636305278 -3.80737065863347
U -1.35538002810681 -2.36257302598845 0.0184936213790798 0.148565425078608 -3.92071568509293
U 1.30917478259643 2.37843864293412 0.0177006045041619 0.148565425078608 -3.94292423774353
l-alanine 0.401502142558538 2.36442096767697 0.0183718656327567 0.148565425078608 -3.97537197099647

U: unknown peaks
Limma result myTopTable_ALL_pad41.tbl.gz