Tutorial of Simple BL-SOM
Finding related genes
In this tutorial, we briefly illustrate how to search for related genes based on transcriptional expression data (Hirai et al. 2004[PubMed] ) using BL-SOM. Hirai et al. analyzed gene expression and metabolite accumulation profiles of leaves and roots under sulfur- and nitrogen-deficiency conditions using cDNA macroarrays and FT/MS, respectively. In this tutorial, we show how to find genes related to glucosinolate metabolism using only the gene-expression profile.
Preparation
Please confirm that you have met the following criteria before you begin this tutorial:
- Have you read the paper ? If you have not read the paper recently, we recommend that you read it again to improve your understanding.
- Have you installed the Java Runtime Environment ? You must install this software on your computer before you can use the Simple BL-SOM software.
- Have you downloaded and installed the Simple BL-SOM software? If not, please download and install the software before continuing. *Simple BL-SOM is no longer available to the public.
Procedure
Procedure (Point-to-point comparison)